ABSTRACT
OBJECTIVE@#To study the application of PCR-SSCP in forensic mtDNA typing.@*METHODS@#Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.@*RESULTS@#In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.@*CONCLUSION@#PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
Subject(s)
Humans , DNA Fingerprinting/methods , DNA Primers , DNA, Mitochondrial/genetics , Forensic Genetics/methods , Haplotypes , Pedigree , Polymerase Chain Reaction/methods , Polymorphism, Single-Stranded Conformational , Sequence Analysis, DNAABSTRACT
<p><b>OBJECTIVE</b>To investigate the polymorphism of DXYS267 locus in China Han population and find the application and characters of its Y-specific single nucleotide substitutions.</p><p><b>METHODS</b>The locus was analyzed by PCR and PAGE in silver-staining. The Y-specific STR was amplified with newly designed primers according to the Y-specific single nucleotide substitutions.</p><p><b>RESULTS</b>Six alleles were detected in Han population in Wuhan. Exact tests demonstrated that genotype frequencies did not deviate from Hardy-Weinberg equilibrium. Heterozygosity of DXYS267 was 0.6706, discrimination power (DP) was 0.8433, and the probability of paternity exclusion (PE) was 0.5957. The Y-specific STR of DXYS267 was successfully amplified with the new primer. The 4 alleles for Y-STR were detected with haplotype diversity (HD) 0.6372.</p><p><b>CONCLUSION</b>The DXYS267 locus is appropriate for individual identification and paternity testing. The new primer is useful for individual and paternity testing involving brothers and mixed stains.</p>